Origin and utility of the reverse dot-blot. Dot blotting A dot blot (or slot blot) is a technique in molecular biology used to detect proteins. Here, I describe a simple protocol to quickly check the presence of recombinant protein in cell cultures using a dot-blot experiment. It represents a simplification of the western blot method, with the exception that the. The blotting procedures can be divided into six main steps 1- Electrophoresis 2- Transfer 3- Blocking 4- Probing 5- Detection 6- Results 1- Electrophoresis The molecule of interest is. 05% Tween-20, pH 7. Dot Blot Handout. Blotting of nucleic acid is the central technique for hybridization studies. Directly dot the extracted RNA onto the nitrocellulose (NC) membrane. M. CONCLUSION 11. 1K views•23 slides. •A northern blot is almost identical to a Southern blot, but it involves the detection of RNA instead of DNA. ppt), PDF File (. W estern blot is the analyti cal technique used in molecular biology, immunogenetics, and other molecular biol ogy to detect specific proteins in a sample of tiss ue homogenate or extract. V. The proteins are then transferred to a membrane (typically nitrocellulose),. 2- Transfer (blotting)- Vacuum Transfer. Open in figure viewer PowerPoint. In Western blotting (immunoblotting) the protein mixture is applied to a gel. DOT BLOT 10. Muhammad Awais. Blotting techniques ppt. 6: Western blot / Immunoblot overview is shared under a CC BY-SA license and was authored, remixed, and/or curated by LibreTexts. Blood Collection, Storage and Transport for HIV-1 Viral Load K2/K3 EDTA Plasma to be separated within 6 hrs Stored at -20 C till further use. Western blot experiments typically include a small number of observations. However, several parameters, such as. 23, and 0. In this presentation the three types of blotting are explained briefly. Dot blotting is an important technique that is routinely used in research and diagnostic laboratories. Irish einzelhandel verzielt wetteinsatzes bis 8% an den emsaatz ëm 4% dot-a slot-blot-ppt -. Rao MD. Methods. 94. Blotting technique including Southern , Northern and Western blotting Rohit Mondal 4K views • 36 slides Northern & Southern Blots presentation. In this regard, the reverse dot-blot method offers a means of screening for several mutations with a single hybridization reaction. It is commonly used by biologists for study of different aspects of protein biomolecules. Dot blot is a simplified procedure in which protein samples are not separated by electrophoresis but are spotted directly onto membrane. The dot-blot assay does not provide any information on the size and number of target nucleic acid molecules. There is also lot of blotting methods like nothern,southern,western blotting. This technique is similar to the western blot technique. JayeshRajput7 •. This dot assay was modified to detect protein by spotting the antigen on a nitrocellulose membrane and incubating the membrane in test antibody followed by incubation in peroxidase-conjugated second antibody to the first antibody, and by. Similarly, this technique had been successfully applied for the detection of mutations related to resistance to RIF and shown a sensitivity of 91. This study indicates that dot-blot hybridization. The blot is incubated with a generic protein (such as milk proteins or BSA) which binds to any remaining sticky. Rinse membrane in 2X SSC. 3 ) Identification by hybridization with a labeled. The double-stranded DNA in the gel is then denatured and transferred to. Sequence alignment Vidya Kalaivani Rajkumar. A levels in total RNA or single RNA species. 68. DNA-binding proteins control various cellular processes such as recombination, replication and transcription. Analysis Expression of ER, (HER-2/neu), and PR with Immunohistochemistry. Dot blotting is a simple technique to identify a known protein in a biological sample. views•. The simplest protein blotting procedure, known as dot blot or slot blot, uses vacuum 1. The appropriate amount of RNA for each dot is between 0. It represents a simplification of the western blot method, with the exception that the proteins to be detected are not first separated by electrophoresis. Dot Blot - an oINSTRUMENTATION & BIOMOLECULAR TECHNIQUE DOT BLOT. BLOTTING TECHNIQUES Definition: Visualization of specific DNA , RNA & protein among many thousands of contaminating molecules requires the convergence of number of techniques which are collectively termed BLOT transfer 1. In a dot blot the biomolecules to be detected are not first separated • Instead, a mixture containing the molecule to be detected is. M. Southern blotting Northernblotting Westernblotting. Western Blotting (also called immunoblotting) is a technique used for analysis of individual proteins in a protein mixture (e. 4 Dot Blotting: Manual Spotting Method 31 1. pressure (a vacuum). pdf), Text File (. Blotting techniques - Download as a PDF or view online for free. Atoms that have same atomic number, but have different masses are known as isotopes. Open in figure viewer PowerPoint. M. • This requires 1 ) Separation of DNA or DNA fragments by agarose gel electrophoresis . 4) for 1 hour at room temperature. T. Western Blot 136659145. The blotting procedures can be divided into six main steps1- Electrophoresis2- Transfer3- Blocking4-. 4. 5K•17 slides. Lectut btn-202-ppt-l23. pptx), PDF File (. Detection of an RFLP by Southern blotting. techniques: •Restriction enzyme analysis •Agarose gel electrophoresis •Hybridization analysis –After electrophoresis, DNA molecules are transferred from the agarose gel onto a filter membrane for probe hybridization. For RNA crosslinking. PCR products were visualized in 1% agarose gels and purified using the GFX PCR DNA and gel band purification kit (GE Healthcare, Buckinghamshire, United Kingdom). The DNA detected can be a single gene, or it can. Dot blot with biotin-labeled DNA using Klenow fragment. Nucleic acid labeling and Hybridization on membranes have. BLOTTING TECHNIQUE p-1. Western blot , une méthode pour des protéines est également une pièce de. M. SOUTHERN BLOTTING Professor Sir Edwin Southern, Professor of Biochemistry and Fellow of Trinity developed this method in 1975. The eastern blot, or eastern blotting, is a biochemical technique used to analyze protein post-translational modifications including the addition of lipids, phosphates, and glycoconjugates. We. TYPES OF BLOTTING TECHNIQUES BLOTTING TECHNIQUES Southern Blot It is used to detect the DNA. Atoms that have same atomic number, but have different masses are known as isotopes. Far Western Blot Technique # 1. Extract & purify DNA from cells 2. The Northern blot, also known as the RNA blot, is one of the blotting techniques used to transfer DNA and RNA onto a carrier for sorting and identification. (2006) using the microfiltration dot blotA Dot Blot Technique . This dot assay was modified to detect protein by spotting the antigen on a nitrocellulose membrane and incubating the membrane in test antibody followed by incubation in peroxidase-conjugated second antibody to the first antibody,. BLOTTING - Blotting techniques are what scientists use to separate these types of molecules. Our results therefore suggest that, direct DNA sequencing may be preferred over reverse dot-blot PCR in critical diagnostic situations like genetic counseling for prenatal diagnosis. , Southern, dot blots, NPA Oligonucleotide End labelling • 3' end labeling of DNA is usually carried out using terminal transferase in template-independent manner to form a homopolymeric tail. Southern blotting is the transfer of. 3. 5. In fact, essential western blot methodology, including sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) [1] and monoclonal antibodies with antigen specificity [2], were only established in 1970 and 1975, respectively. Definition: Non fractionated or non-electrophoresed samples are directly blotted and immobilized on a nitrocellulose or nylon membrane as dots or spots for hybridization. Western blotting, also called immunoblotting, is a widely used and accepted technique to detect levels of protein expression in a cell or tissue extract. Used to identify specific molecules in a complex mixture of related molecules. Title: Northern blotting 1 DNA (or RNA) samples loaded into wells 2 (No Transcript) 3 Ethidium bromide is fluorescent in UV light 4 Northern, Southern, Western In the 1970s Ed Southern of Oxford University invented a revolutionary DNA blotting technique. If the radioactive label is not used, the bound antibody. Denature DNA 5. Blotting technique is the technique in which nucleic acids or proteins are immobilized onto solid support generally nylon or nitrocellulose membranes. ppt. 2, 2019. individuals of each family were applied to dot blot assay with the optimized conditions. 5K views•30 slides. pdf), Text File (. A technique named eastern blotting was developed for the specific and easy identification of BI in the extracts of crude drugs and KMs using anti-BI monoclonal. Hybridization: It is the process of forming a. Blotting techniques Dr. 1-HISTORIQUE Northern blot est une technique d'ARN blot qui a été développé en par Alwine, Kemp, et Stark en 1977 à l'Université Stanford . Southern Blot It is used to detect DNA. ppt Sadia Noreen 11 views • 19 slides Similar to. Western blotting pppt sworna kumari chithiraivelu. Blotting techniques generally involve antigen detection on the surface of a membrane. Thus, Eastern blotting can be considered an extension of the biochemical technique of Western blotting. A screening test, comprising. f IMMUNOASSAY. Nucleic acid labeling and hybridization on membranes have formed the basis for. TYPES OF BLOTTINGTYPES OF BLOTTING. For β- thalassemia mutations, the reverse-dot-blotting technique, in which amplified DNA is hybridized to a panel of mutation-specific probes fixed to a nylon strip has been widely used. 8K views• 13 slides. F. Protein blotting is an analytical method that. The southern blotting technique is used for the detection of DNA. This review is aimed to summarize some of the most commonly used techniques to determine DNA–protein interactions. that is. The transferring medium then goes through several chemical and physical treatments. 1. In recent years, far–western blot. Ashish Patel, Ph. A blot resulting from 1-dimensional. 1K views•31 slides. 3. secondary probe is biotinylated anti-immunoglobulin. Abstract. The processes for each are similar, involving gel electrophoresis, transfer to a membrane, and hybridization. 1. •Four steps: –DNA extraction –Electrophoresis to separate –Transfer to membrane –Use labeled probes, which will hybridize to specific sequence, to identify sequence of interestA Southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples. • After incubation, the protein bands (immune complexes) are visualized with peroxidase-conjugated protein and a colour reagent. Due to the simplicity of the technique it widely used as a ideal diagnostic tool. 5. The in-house reverse dot blot hybridization (RDBH) assay has been widely used in the spoligotyping technique for M. Physiol. materi Teknik pelacakan molekuler-genomik. Mengidentifikasi semi kuantitatif dari reaksi antigen. Western blotting techniques Southern blotting Northern blotting Western blotting Molecule detected DNA (ds) mRNA (ss) Protein Gel electrophoresis Agarose gel Formaldehyde agarose gel Polyacrylamide gel Gel pretreatment Depurination, denaturation, and neutralization - - Blotting method Capillary transfer Capillary transfer Electric transferDot blot has several uses. Nevertheless, with the development of fast blotting systems. PDF | On May 1, 2000, David I. It follows a similar principle to Western blotting, except the sa. Analytes in biological liquids such as. A cross indicates an incorrectly applied sample that was excluded for the analysis. 5 μl), the solution can be applied directly with a capillary micropipette. 5: Practical Applications of Monoclonal and Polyclonal Antibodies. Dot Blot is a cheaper, easier and faster technique to detect the presence of Proteins and Nucleic Acids in a biological sample. Types of blotting techniques: • Southern blotting • Northern blotting • Western blotting • Colony blotting. linked to the membrane. The hybridization procedure is the same as in original blotting techniques. BLOTTING • A molecular technique of transferring proteins, DNA or RNA, onto a carrier or membrane. B Western Blotting. Southern Blotting 2. 1. Be able to explain the various types. Types of blotting techniques. 6. WESTERN BLOTTING 4. Blotting techniques includes southren,northern,western and dot blotting. WESTERN BLOTTING Western blotting, also known as Immunoblotting or Protein blotting, is a core technique in cell and molecular biology. 50. Your sample could be tissue, cells, or another solution that you want to extract and analyze its protein. Despite the availability of a wide range of commercial kits, protein quantification is often unreliable, especially for tissue-derived samples, leading to uneven loading in subsequent experiments. If a sample contains specific antibodies, these bind to the membrane-bound antigens. Dot blotting A dot blot (or slot blot) is a technique in molecular biology used to detect proteins. Blotting (Southern, Northern and Eastern) Aman Ullah 27. Southern blotting Akansha Soni. Rajanathan. To validate the application of ELISA and slot blot techniques in protein–protein studies, we employed these two techniques to explore the interaction of recombinant PfHsp70-1 with its partners. Southern Blot It is used to detect DNA. In vitro techniques such as footprinting assays, electrophoretic mobility shift assay, southwestern blotting, yeast one-hybrid assay, phage display and proximity. Technique # 1. B. Dot Blots. The chromogenic substrate is added to a. Yet, it still alludes to it in its name, reaffirming the popularity of the western blot. If the volume to be added to the membrane is small (ca. Although this technique is quite labor-intensive, it's particularly useful for detecting large deletions in tumor genomes. Figure 4. System. This technique is helpful in detecting the. of MBB&B, CCS Haryana Agricultural University, Hisar Lecture Title: Techniques used in Molecular Probing Agarose or Polyacrylamide gel electrophoresis • When genomic DNA extracted from any tissue of a plant or animal species is digested with a restriction enzyme, it is cleaved into segments. 8% for examination of H&E-stained tissue sections. Neurologic Evaluation and Ancillary Tests. Kashyap Kashyap. Western blotNorthern Blot It is used to detect RNA. 3 Reverse Dot Blot. Southern Blot. •The method is named after its inventor, the British biologist Edwin Mellor Southern,In 1975. Southern blotting combines transfer of electrophoresis -separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization. Dot blotting (DNA/RNA) The Southern blotting is named after the scientist Ed Southern (1975) who developed it. they allow a higher degree of labeling to be achieved than end-labeling techniques. , 60 (Suppl 3) (2009), pp. B Used To Identify A Specific RNA . Southern Blot. To oversimplify, DNA molecules are transferred from an agarose gel onto a membrane. Dot-blotting technique is particularly useful in obtaining quantitative data for the evaluation of gene expression. Blotting technique has become widely used for specific identification of nucleic acid and proteins. It is similar to western blotting, but does not require separation by electrophoresis. What is blotting? Blots are techniques for transferring DNA , RNA and proteins onto a carrier so they can be separated, and often follows the use of a gel electrophoresis. The protein once immobilized on the nitrocellulose membrane can be probed with a primary antibody specific for the protein of interest. The technique was discovered by Edwin. Eastern blot is primarily performed to detect the. • The process of transfer of the denatured fragments out of the gel and onto a carrier membrane makes it accessible for analysis. 45, 1. 82. The dot blot procedure is a well-validated technique, and its use is an alternative to avoiding the protein separation process although the equipment available. Types of blotting techniques 1 ) Southern blotting ( to detect DNA ) 2 ) Northern blotting ( to detect RNA ) 3 ) Western blotting ( to detect protein )Dot Blot protocol technique for detecting, analyzing, and identifying proteins, similar to the western blot technique but differing in that protein samples are not separated. Western Blotting - Download as a PDF or view. The technique is a modified version of the Southern Blotting, which was discovered for the analysis of DNA sequences. However, it can only be applied to animals that bear a. , 2018 ). Electrophoresis 23, 2373–2376 Kakita, K. They have been developed to be highly specific and sensitive and have become important tools in both molecular biology and clinical research. doi: 10. 5M NaCl) 10mL 10% Tween 20 890mL Deionized Water 1x TBST Buffer + 5% Milk (1L) Note: Powdered non-fat dry milk must be thoroughly dissolved into buffer before Tween-20 addition soJul 3, 2023. The mean BrdU incorporation was 100 ± 15, 10 ± 6, and 278 ± 98% in the control, fasting, and refeeding group, respectively ( Fig. 3. This method is used in molecular biology. WESTERN BLOTTING • The Western blot is an analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract. In this regard, the reverse dot-blot method offers a means of screening for several mutations with a single hybridization reaction. It is relatively simple to perform, inexpensive and not. The technique is known as DNA transfer or 'Southern blotting' Professor Sir Edwin Southern. Samples can be in the form of tissue culture supernatants, blood serum, cell extracts, or other preparations. 4, 1. Dot blot refers to the deposition of a protein solution directly onto the membrane (20 ). 5 M NaCl, 0. • In this approach, the Nucleic acids (DNA or RNA) are. tuberculosis lineage identification. 2 A ). Dot Blotting, SDS-PAGE and Western Blotting. HISTORY 2. A second incubation with conjugated antibodies specific to the primary antibodies (secondary antibodies. Abstract. 2 ) DNA fragments are blotted onto a strip of nitrocellulose or a nylon membrane. A quantity of. This protocol provides the general workflow to simply entrap nucleic acids (in this case DNA) onto a Nylon membrane [ 1 – 3 ]. This technique is the first nucleic acid technique that is developed in 1975 by E. Western blotting (for Protein) 4. For example,. carrier or membrane. ; The detection of certain sequences of nucleic acids extracted from different types of biological samples is. Similarly, this technique had been successfully applied for the detection of mutations related to resistance to RIF and shown a sensitivity of 91. Western blotting • Western blot is the analytical technique used in molecular biology, immunogenetics and other molecular biology to detect specific proteins in a sample of tissue homogenate or extract. 23. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. Blotting technique. Blotting of nucleic acids is the central technique for hybridization studies. TYPES OF BLOTTING TECHNIQUES Blotting technique Southern Blot It is used to detect DNA. Umair Ahmed Submitted by: Aymen fareed Roll No: PBOT014 DEPARTMENT OF BOTANY What is blotting technique Blotting Types of blotting Mechanism of southern and western and northern blot blotting Steps all blotting membrane. Electrophoretic as well as non-electrophoretic transfer of proteins to membranes was first described in 1979. KhushiDuttVatsa • 34. The dot blot method is a qualitative assay, which utilizes strips of nitrocellulose on which purified antigens are blotted at pre-located spots. Human leukocyte antigen (HLA)-DQα1 gene found on chromosome-6 was the first commercially available PCR-based forensic DNA fingerprinting kit. Western blotting involves the transfer of proteins that have been separated by gel electrophoresis onto Dot-blot hybridization provides a simple, easy, and fast way to quantify the relative concentration of ANF mRNA within cardiac tissue. PEP ppt. (A) Reverse dot-blot analysis for a family (Bo: father, Me: mother and Con: children). Blotting. Western blotting is an important technique used in cell and molecular biology. 8% for examination of H&E-stained tissue sections. Western blotting is the counterpart which is used to detect proteins. • After incubation, the protein bands (immune complexes) are visualized with. J. They involve identification of protein target via antigen-antibody (or protein-ligand) specific reactions. Southern blotting. It is used to. Radiolabeling Technique Radiolabeling (or isotopic labelling) is a technique used to track the passage of an isotope (an atom with a detectable variation) through a reaction, metabolic pathway, or cell, called radiolabeling technique. Blots are techniques for transferring DNA , RNA and proteins onto a carrier so they can be separated, and often follows the use of a gel electrophoresis. Incubate the membrane for 1 hour at room temperature. • Since, Nucleic acid hybridization is a process used to identify specific DNA sequences. Dot blot analysis is an important method to identify a known protein in a sample. • The segments of different sizes can. Saba Ahmed M. Blotting Techniques Kurgat Gilbert 160. Southern. 4. Blotting is used for a selection of recombinant plants . Dot Blot protocol technique for detecting, analyzing, and identifying proteins, similar to the western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane or paper substrate. Take the membranes out of the plastic bins. Western blot (WB) or immunoblot is a workhorse method. 5 Blotting technique. Dot and slot blot ppt . Briefly, DNA is fragmented by incubation with restriction endonucleases and the fragments are then separated by electrophoresis. DOT BLOT TECHNIQUE This technique is used to detect the presence of a given sequence of DNA/RNA in the non- fractionated(not subjected to electrophoresis). Cell as basic unit of life ppt 88 slides. Fijar el ADN al soporte mediante calor o UV y continuar con do blot tradicional. It is most often used to detect carbohydrate epitopes. Use of the dot blot technique to estimate degree of relationship between strains of a virus. Southwestern Blotting Assay. Western blotting: Western blotting is an Immunoblotting technique which rely on the specificity of binding between a molecule of interest and a probe to allow detection of the molecule of interest in a mixture of many other similar molecules. METHODOLOGY A general dot blot protocol involves spotting 1–2 microliters of a samples onto a nitrocellulose or Polyvinylidene difluoride (PVDF) membrane and letting it air dry. Principle of Southern Blotting Southern blotting is a hybridization technique for identification of particular size of DNA from the mixture of other similar molecules. • A Dot blot (or Slot blot) is a technique used to detect biomolecules. Cont…. 1586/14737159. Immunoprecipitation: Procedure, Analysis and Applications ajithnandanam. Southern blotting (for DNA) 2. M. The other names Northern blotting and Western blotting areTYPES OF BLOTTING TECHNIQUES w 4 Blotting technique Western blot It is used to detect protein Northern Blot It is used to detect RNA. Blotting Techniques PPTSouthern blotting is used to assess for. Southern blotting is a process that transfer the DNA fragments that separated by electrophoresis into a nitrocellulose membrane/ nylon. The difference lies in the visualization process. Define dot blot technique. To be done by personnel trained in DBS collection technique. Therefore, the first step in combating viral pathogens is to get a timely and. They have been used in various fields to assay for a manifold of a constituent from nucleic acid and other complex proteins [127,137,138]. Mark the dot position and front side of the membrane. Here we show that the widely used Bicinchoninic Acid (BCA) assay tends to underestimate protein concentrations of tissue samples. SOUTHERN BLOTTING 5. 5K• 31 slides. In DB or SB the sample is applied directly to the membrane as dots or as slots using a vacuum apparatus. Sample sizes in blot experiments are rarely large enough to justify. First, it offers a quick and easy method for checking whether a sample contains a particular protein. Moreover, the simplicity of conducting the northern analysis experimentally as well as its economic feasibility has globally propelled this technique as the method of. 3. 2 In these techniques nucleic acid hybridization is used to detect and quantify specific DNAs/RNAs. If the volume to be added to the membrane is small (ca. The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. Immunoblotting Saranraj P. Serial dilutions of HeLa cell lysate (7. Remember to always carefully consider the purpose of your experiment before you start. Southern, the technique of Southern blotting is one of the most important methods used in molecular biology. Eastern blot was discovered as an extension of the more popular western blotting technique. 4. Dot Blotting; SOUTHERN BLOTTING. Autoradiograph showing the extent of sequence similarity between MSV-MNM and other MSV strains. The Southern blot is used for transferring DNA, the Northern blot for RNA and the western blot for PROTEIN. The probe hybridizes to its complementary sequence and the hybridization product is detected with a. D. Detection of the sickle-cell globin gene by Southern blotting. This enables radiolabeled or enzymatically labeled. Types of blotting techniques: • Southern. Techniques of precipitating antibodies to ENA were discovered and used as diagnostic tools in CTD almost 5 decades ago . 1K views•31 slides. . The techniques of Southern and Northern blotting have become widely known and are extensively used in the study of molecular genetics. 18. Number. , 1979), protein blotting has become a routine tool in research laboratories. BLOTTING TECHNIQUES. Scholar, Animal Genetics & Breeding. Keywords: Alpha chain; deletion; molecular techniques; non-deletion; thalassemia. If simply checking for presence of your POI, then the dot blot is the way to go as it will save you time and materials. 3% comparing to. Whether the DNA sequence is present or not. Title: Northern blotting 1 DNA (or RNA) samples loaded into wells 2 (No Transcript) 3 Ethidium bromide is fluorescent in UV light 4 Northern, Southern, Western In the 1970s Ed Southern of Oxford University invented a revolutionary DNA blotting technique. Prasad Naidu 135. This is Part B, Blotting Techniques, under the module topic, Nucleic Acid Hybridization & Expression Analysis. 25. Instead, the sample is applied directly on a membrane in a single spot, and the blotting. The technique is actually very similar to the Western blot, but dot blot, for. Blotting techniques Dr. Blotting techniques includes southren,northern,western and dot blotting bbmy. • Allow to identify a particular protein of interest. It is used to detect DNA. 2 Visualization by Reversible Staining 34Southern Blot. The second strip precludes the need for more technically challenging methods. 4. Southern, northern, and western blot protocols are similar, and begin with electrophoretic separation of protein and nucleic acid fragments on a gel, which are then transferred to a membrane (nitrocellulose membrane, polyvinylidene difluoride (PVDF) membrane, etc. Blotting Techniques A dot blot (or slot blot) is a technique in molecular biology used to detect biomolecules, and for detecting, analyzing, and identifying proteins. b Dot blot assays were set up with raised rabbit anti-BEFV or negative control antiserum. botany unit 2. The remaining of this article is focus on different techniques and applications. Fluorescent blotting is a newer technique and is growing in popularity as it affords the potential to multiplex (detect multiple proteins on a single blot). While both techniques are used to identify nucleic acid sequences, Northern blotting is performed to detect RNA sequences, while Southern blotting is done to detect DNA sequences. Southern Blotting Southern blotting was named after Edward M. See full list on biologyreader.